Computational gene finding obtains increasing concerns in the metagenomic sequencing projects. Accurate prediction of gene on both 5'-end and 3'-end is important for experimental identification of purified protein products, as well as predictions of promoter and operon. However almost all of the current algorithms are inapplicable to accurately locate the translation initiation sites (TISs) of protein coding genes; they are usually demonstrated a poor performance on experimentally verified gene data. To address this problem, we provide MetaTISA, a tool that post-processes predictions from gene-finders for metagenome with an aim to improve the prediction of TISs.

At this moment, it provides option for MetaGene (Noguchi et al., 2006, 2008) and will extend to other gene finders in later developments.

To facilitate the study of diversity of translation mechanisms among different environments, we provide a matlab script to visualize the positional weight matrix generated for each binned cluster.

Online Prediction

Download stand-alone MetaTISA (Linux/Windows).

A webserver for TIS prediction in complete microbial genomes TiCo

A database for TIS annotation for microbial genomes ProTISA

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